… Another highly specific and sensitive molecular method is nested RT-PCR (nRT-PCR)
which involves 2 rounds of PCR amplifications. As previously reported, the lowest …

… obtained from avian influenza and Newcastle disease-free … Nested PCR was performed in
a mixture of 20 μL containing … RTPCR assay for the detection of infectious bronchitis virus from …

… The avian influenza virus (AIV), Newcastle disease virus (NDV), and infectious bronchitis virus
… Many studies have also been performed using multiplex real-time RT-PCR to differentiate …

… chain reaction (RT-PCR) and its variants (multiplex, nested, … , are routinely used for detection
and genotyping of IBV strains [… , avian infectious laryngotracheitis virus, avian influenza virus…

… for the first time when avian influenza broke out in Asia in … RT-PCR, nested RT-PCR (nRT-PCR),
quantitative RT-PCR (qRT-… tools for detecting NDV and have the potential for a multiplex …

… , 40 were class I Newcastle disease virus (NDV or APMV-1) … avian influenza viruses (AIVs),
one infectious bronchitis virus… of semi-nested or nested RT-PCR assays targeting the viral L …

… RT-PCR and was comparable to real-time RT-PCR assay. The developed assay was found
to be specific as it did not detect closely related infectious bronchitis virus … multiplex detection…

… To date, ten respiratory pathogens (viruses and bacteria) have been … multiplex PCR, including
avian influenza virus (AIV), infectious bronchitis virus (IBV), Newcastle disease virus (NDV)…

… obtained from avian influenza and Newcastle disease-free … Nested PCR was performed in
a mixture of 20 μL containing … RTPCR assay for the detection of infectious bronchitis virus from …

… amount of viral RNA can be detected by nested RT-PCR. … diagnosis of Newcastle disease,
IB, and avian influenza based on … -step multiplex real-time RT-PCR for simultaneous detection …